After treatment with a concentration gradient of shikonin, HCT116 (Figure 1A-1B) and SW480 (Figure 2C-2D) cells were induced a marked increase in the proportion of early (Annexin V+, PI-) and late apoptotic (Annexin V+, PI+) cells, and there were almost no necroptotic (Annexin V-, PI+) cells in HCT116 and SW480 colon cancer cells. Here, ANXA5 is linked to colonic neoplasm.