The averaged FI signal of CXCR4 protein in live BC spheroids increased from 1.98±2.3au to 4.24±5.0 (p<0.0013), and the area fraction of the receptor signal increased from 7.8±7.1 au, to 16.4±18.1, p<0.0008, following NO mediated migration (Figure 10B). The gene discussed is CXCR4; the disease is breast cancer.