TGFB1 and hepatocellular carcinoma: Further Dual-Glo-luciferase analysis demonstrated that curcumin significantly suppressed pGL3-Basic-Snail-luc activity which enhanced by TGF-β1 in hepatoma cells (Figure 6D), which is in accord with the results that curcumin inhibited the mRNA and protein expression of Snail (Figure 5).