They initially found that simultaneous overexpression of the main importer (dZip42C.1) and silencing of the main exporter (dZnT63C) triggered Zn entrapment in the cytosol, that lead to a Zn toxic phenotype (named Ztox) characterized by either retinal degeneration and loss of eye pigmentation when carried out in the developing eye (gmr-GAL4) or by scutellum loss, thorax cleft, hypopigmentation and bristles misaligned when performed in the developing thorax (pnr-GAL4) (Lye et al., 2012). This evidence concerns the gene LGALS4 and retinal degeneration.