The six candidate NRRS genes were screened for the presence of cis-regulatory elements (Table 3) that have been experimentally validated to play a role in root-specificity i.e., AS1, Sorlip1, and FaRB7, and typical for promoters of genes that are upregulated in nematode feeding site (NFS) upon infection i.e., TobRB7 box A/B etc. The purpose of this regulatory element based screening was to further reduce the number of genes selected for downstream molecular cloning and in planta validation. Here, PTGDR is linked to infection.