We focused on the eight HTNV GP nonapeptides that exhibited a high-binding affinity to the HLA-A*0201 molecules, and utilized them to synthesize peptide/HLA-A*0201 tetramer complexes to examine whether the eight nonapeptides could be recognized by the TCRs of CD8+ T cells in HLA-A*02+ patients with HFRS. The gene discussed is HLA-A; the disease is hemorrhagic fever with renal syndrome.