Even though significant difference in the multifunctional response was observed in CD4+ and CD8+ T cells between LdCen−/−2W (GIII) and LdWTLLO + LdCen-/-2W (GI) groups, there was no significant difference in splenic parasite burdens between these two groups suggesting that once a certain threshold T cells response is achieved it can control parasitemia. This evidence concerns the gene CD4 and parasitic infectious disease.