The strongest defensive efficacy in the mice models (C57BL/6 and BALB/c) against infection to L. major was observed by Zahedifard et al., when they primed these animals with PpSP15 DNA and boosted them with the combination of PpSP15 DNA and L. tarentolae (live) that was engineered to stably express genes for CPs (52). This evidence concerns the gene CAD and infection.