Subsequently, we detected the effect of the conditional medium derived from IDH1wt U251 Tet-on-LRRC4 cells on CD4+CCR4+ T cells, Ti-Treg cells and Ti-Teff cells and obtained results that were consistent with those obtained for primary cultured GBM cells (Figures S1C–F in Supplementary Material). This evidence concerns the gene CCR4 and glioblastoma.