The contribution of the NLRP3 inflammasome in hepatocytes was further supported by Csak et al., who demonstrated that saturated fatty acids (FAs) represented an endogenous danger signal as a first hit, triggered NLRP3 inflammasome activation in mouse models of MCD diet-induced and high-fat diet-induced NASH, and ultimately induced sensitization to a second hit with LPS for the production of cytokine IL-1β in mouse hepatocytes (41). Here, NLRP3 is linked to metabolic dysfunction-associated steatohepatitis.