We have shown earlier how Gli3Xt−J/Xt−J mice, which produce no functional GLI3 protein, can be used as a model to study craniosynostosis and suture biogenesis, and by genetically reducing the dosage of Runx2 the calvarial phenotype can be rescued (Rice et al., 2010; Tanimoto et al., 2012; Veistinen et al., 2012). Here, GLI3 is linked to craniosynostosis.