To further confirm these results, the mitochondrial permeability transition inhibitor (cyclosporine A; CyA) was used prior to treatment with BD in PANC-1 and Capan-2 cells, and the expression of several mitochondrial-associated apoptotic proteins, such as cytosolic cytochrome C, Bax and Bcl-2, was analyzed by Western blotting. The gene discussed is CYCS; the disease is Behcet disease.