To explore the effects of physical exercise on the CX3CL1/CX3CR1 axis and its downstream MAPKs signaling in rats with CCH, CX3CR1/Iba1 immunofluorescence double-labeled staining was performed to observe the expression of the CX3CR1 specifically in microglia at 28 days after 2VO (Figure 6G). The gene discussed is CX3CL1; the disease is columnar cell hyperplasia of the breast.