In our study, one of the limitations found was that the effect of leptin on cell proliferation and expression of AdipoR1 and AdipoR2 was modest, due to the range of leptin concentrations used to perform the cellular stimulus was very wide, which did not allow to identify exactly the effect that this protein has on the gene expression of these receptors in a state of obesity. This evidence concerns the gene LEP and obesity due to melanocortin 4 receptor deficiency.