Gene set enrichment analysis (GSEA) showed that CASC9.5 expression was related to tumor metastasis, tumor microenvironment and cyclin genes (Fig. 6D).Therefore, western blotting was performed to examine the expression level of E-cadherin and 2 other EMT markers (N-cadherin and β-catenin) in CASC9.5-KD A549 cells. This evidence concerns the gene CDH1 and neoplasm.