HSV-1EdU or ΔICP0EdU infection of shCtrl cells recapitulated observations made in parental HFt cells (Fig 3, S3 Fig, S4 Fig), demonstrating that lentiviral transduction, shRNA expression, or puromycin selection did not affect PML-NB entrapment of vDNA or alter IFI16 localization (Fig 4C–4E). This evidence concerns the gene PML and infection.