The results showed that the average methylation of the CRMP4 promoter occurred at 9 sites (methylation sites − 680, − 678, − 674, − 671, − 665, − 660, − 658, − 848, and − 841) in 10 cases of PMLP, 20 cases of prostate adenocarcinoma, 10 cases of LPCa-LN (−) and 10 cases of mPCa-LN (+) and were 42.3, 30.6, 6.7, 20.3%, respectively. The gene discussed is DPYSL3; the disease is prostate adenocarcinoma.