Western blot analysis showed a downregulation of dnmt3b in miR-494-overexpressing Huh-7 cells and an upregulation in anti-miR-494-transfected SNU182 cells (Fig. 2j), chosen based on miR-494 basal levels (Supplementary Fig. S3A), demonstrating DNMT3B as a miR-494 direct target in HCC. Here, DNMT3B is linked to hepatocellular carcinoma.