PTEN and hepatocellular carcinoma: The reporter assay showed a decreased luciferase activity of wild type, but not mutant (Supplementary Fig. S3B, D), 3'UTR-vectors in miR-494 co-transfected with respect to control HepG2 cells (t-test; p < 0.05) (Fig. 3e), demonstrating PTEN, P27 and PUMA as miR-494 direct targets in HCC.