GOLPH3 and infection: Mixed Agrobacterium cultures carrying the binary pK2GW7:pCas13a clones, TRV RNA1, and the engineered TRV RNA2 genome harboring crRNAs against one of the four different TuMV-GFP genome targets (HC-Pro, coat protein [CP], GFP target 1 [GFP1], GFP target 2 [GFP2]), as well as TuMV-GFP infectious clones co-delivered into N. benthamiana leaves via agro-infection.