After challenge infection, the immune profile was maintained in the vaccinated group mice, since higher levels of protein and parasite-specific IFN-γ IL-12, and GM-CSF were found, while in the saline and saponin groups, animals produced higher levels of IL-4 and IL-10, which proved to be compatible with the development of the Th2 response (Figure 4a). This evidence concerns the gene IL4 and infection.