At last, we integrated all of the above genes related to esophageal cancer and identified 21 non-redundant genes that were related to esophageal cancer, among which seven genes have been confirmed to be biomarkers of esophageal cancer by previous research, and six genes (RBPMS2, PDK4, IGK, SBSN, IFIT3 and HSPB6) may be novel biomarkers for the diagnosis of esophageal cancer. The gene discussed is PDK4; the disease is esophageal cancer.