It was demonstrated that HDGF knockdown decreased the expression of the mesenchymal markers Vimentin and N-cadherin, and the EMT central regulators Snail and Slug compared with shRNA-CTR transfected cells or PBS-treated cells, while the expression of epithelial marker E-cadherin was upregulated in PCa cell lines DU145(Fig 5A and 5B), PC3 (Fig 5C and 5D) and LNCaP (Fig 5E and 5F) following HDGF knockdown. This evidence concerns the gene CDH2 and posterior cortical atrophy.