Consistently, we also found that FR5 treatment decreased the expression of Bcl-2 based on immunohistochemical analysis of the Bel7402 xenograft tumors (Figure 8C), which suggested that the enhanced apoptosis of HCC cells by FR5 treatment may be due to the activation of mitochondrial apoptotic pathways. This evidence concerns the gene BCL2 and hepatocellular carcinoma.