Collectively, these results implied that FR5 induced anti-cancer effect through coordination between the Hippo and PI3K/AKT pathways, and this is executed at least in part through the actions of YAP and PTEN, which subsequently affects AKT, resulting in the eventual decrease in cell proliferation and increase in cell apoptosis on HCC cells. This evidence concerns the gene AKT1 and hepatocellular carcinoma.