Collectively, these results implied that FR5 induced anti-cancer effect through coordination between the Hippo and PI3K/AKT pathways, and this is executed at least in part through the actions of YAP and PTEN, which subsequently affects AKT, resulting in the eventual decrease in cell proliferation and increase in cell apoptosis on HCC cells. The gene discussed is YAP1; the disease is hepatocellular carcinoma.