In order to extensively explore the relationship of MTHFR SNPs with LC susceptibility, we selected MTHFR tagging SNPs (rs3753584 T>C, rs4845882 G>A, rs1801133 G>A, rs4846048 A>G and rs9651118 T>C) and carried out a case-control study to determine the potential effect of MTHFR SNPs on NSCLC risk. This evidence concerns the gene MTHFR and non-small cell lung carcinoma.