Clinicopathological parameters like tumor differentiation, age, vascular invasion, TNM staging, survival rate, tumor position and the expression of Ki-67 and P53 were statistically different among the subgroups divided by the 16 serum metabolites selected from the PLS-DA results by the using chi-square test or Kaplan-Meier (K-M) survival curve (p<0.05) (Figure 6). The gene discussed is MKI67; the disease is neoplasm.