We did not find any significant association between NIS expression in the membrane and age, tumor size, tumor capsule, multifocality, lymphocytic infiltration, vascular invasion, lymph node metastases, tumor margins, distant metastases, staging, BRAF, NRAS and TERTp status, additional treatments, disease-free status at one year, disease-free status at the end of follow-up or disease-specific survival in the DTC group. This evidence concerns the gene NRAS and metastatic malignant neoplasm in the lymph nodes.