FACS analysis was performed to compare the cytotoxic activities of CTLs induced by unstimulated DCs (DC-CTLs), TL-CTLs (MCF-7 Tumor cell lysate stimulated DC-CTLs), Sorting-CTLs (CD44+ MCF-7 cell lysate stimulated DC-CTLs) and Pep-CTLs (which were induced by DCs stimulated with one of the following peptides: CD44-1, CD44-3 or CD44-6) against CD44+ MCF-7 cells. The gene discussed is CD44; the disease is neoplasm.