Thus, the present study investigated whether NETO2 expression in CRC was correlated with that of key genes involved in the EMT, including TWIST1, SNAIL1, SNAIL2, ZEB1, ZEB2, LEF1, FOXA2, FOXA1, CDH1, STAT1, BMP2, BMP5, VIM, SMAD2, SMAD3, SMAD4, and SMAD7. The results obtained in the work confirmed that NETO2 is overexpressed in CRC. This evidence concerns the gene SMAD4 and colorectal carcinoma.