To investigate whether MARCH8 plays a role in NSCLC growth through apoptotic regulation, fluorescent-activated cell sorting (FACS) was performed to detect Annexin V and Propidium Iodide (PI) positive cells in MARCH8 and MARCH8-KD groups of A549 and H1299 cells. The gene discussed is MARCHF8; the disease is non-small cell lung carcinoma.