To examine whether NCOA5 facilitates CRC cell proliferation through directly accelerating cell cycle progression, flow cytometric analysis was applied to detect the cell cycle distribution in shNCOA5 2#- or shNCOA5 3#-transduced vs shNTC-transduced SW620 cells, and LV-NCOA5-transduced vs LV-transduced SW480 cells. The gene discussed is NCOA5; the disease is colorectal carcinoma.