Intriguingly, knockdown of YAP1 in purified small CRC cells (i.e., LoVo and HT-29 cells) inhibited holoclone-forming (Figure 5D and 5E) and sphere-forming capacity (Figure 5F and 5G) while there was no significant difference upon knocking down of YAP1 in purified large CRC cells. This evidence concerns the gene YAP1 and colorectal carcinoma.