In contrast, genes linked to metabolic and intracellular signaling pathways (i.e: the fatty acid, retinol, glycerophospholipid, GnRH, arachidonic acid and the phosphatidylinositol) related to inflammation [25–27] and cell migration [28, 29], were down-regulated among MTX vs. non-MTX sCRC primary tumor samples. This evidence concerns the gene GNRH1 and neoplasm.