Q-RT-PCR of RNA isolated from FACS-sorted monocytes, macrophages, neutrophils, and HER2+ cancer cells during early stages of progression confirmed the CCL2 production by HER2+ early lesions (Supplementary Fig. 5J, Supplementary Table 7), as detected by q-RT-PCR, IF analysis, and ELISA (Fig. 6b–d, Supplementary Table 6). This evidence concerns the gene CCL2 and cancer.