Previous studies revealed that most of the beta-globin (HBB) gene mutations (>95%) in Bangladeshi thalassemia patients including three most common mutations, namely c.79G > A, c.92 + 5 G > C and c.126_129delCTTT account for ~85% of the beta-globin gene mutant alleles and these mutations are confined in the exon-1 (c.1 – c.92), the intron-1 (c.92 + 1 – c.92 + 130) and a portion of exon-2 (c.93 – c.217), thus constituting the hot-spot region (Table 3 and Fig. 1). Here, HBB is linked to thalassemia.