For instance, the screening of beta-thalassemia carrier which is based solely on hematological or electrophoretic indices may miss a significant number of carriers due to adequacy of mild variant c.79G > A and complex inheritance of other factors such as presence of α-globin gene mutations, mutation in Krüppel-like Factor 1 (KLF1) gene, promoter mutations of gamma-globin gene, iron deficiency anemia, hereditary persistence of fetal Hb etc. [15]. This evidence concerns the gene KLF1 and Iron deficiency anemia.