To test this, cells were pretreated with 100μM of the cell permeable Ca2+ chelator BAPTA-AM, or the non-permeable chelator, BAPTA prior to infection, and the ability to detect PtdS, Akt, or PLSCR1 at the outer leaflet was monitored by confocal imaging 30 min following infection. This evidence concerns the gene PLSCR1 and infection.