The addition of R5421 at the time of infection (t = 0) prevented PtdS and Akt externalization, whereas the addition of R5421 30 min pi resulted in persistence of PtdS and Akt at the outer leaflet for up to 4 h pi (Fig 7E), indicating a role for PLSCR1 in the bidirectional movement of PtdS and Akt during HSV entry. The gene discussed is PLSCR1; the disease is infection.