We carried out validation studies to assess the importance of the prioritized GLP-1 response subnetwork through associations with: i) GLP-1 stimulated insulin secretion in an independent collection of 100 unrelated individuals from Tübingen, Germany[10], ii) glucose stimulated insulin secretion phenotypes in up to 5,318 individuals from MAGIC [11] and iii) gene expression alterations in white adipose tissue as a response to liraglutide (a GLP-1 receptor agonist) treatment in ob/ob mice (a mouse model of obesity). Here, GCG is linked to obesity due to melanocortin 4 receptor deficiency.