INSR and obesity due to melanocortin 4 receptor deficiency: To confirm the above data in the context of human obesity, we next isolated EVs from plasma obtained from lean and obese women (Supporting Information Table S1) using previously established ultracentrifugation protocols.19 EV samples were analyzed by western blotting to confirm the presence of EV markers (CD81, MHCI) and the absence of other cellular membrane markers (insulin receptor, GRP78) and were compared to EVs released by 3T3‐L1 adipocytes (Figure4A).