Following differentiation of cells into adipocytes, cells were incubated in DMEM for 24 h to remove hormones and subsequently treated with EVs from either lean women or women with obesity at equal concentration (2 × 105 EV mL–1) as determined by NTA, and we assayed 2‐deoxyglucose uptake in response to insulin as described previously.16 Transport was normalized to cellular protein content. This evidence concerns the gene INS and obesity due to melanocortin 4 receptor deficiency.