CDH23 and infection: First, we observed that the real-time qPCR amplification using primers D (located at the 3′ end of vector 2) and E (located at the 5′ of vector 3) following infection with AAV 2 + 3 is significantly lower than the one obtained when AAV 1 + 2 + 3 are used (2% for CDH23 and 20% for ALMS1).