Similarly, pro-IL-1β protein levels significantly increased after T. gondii infection in a time-dependent manner, peaking at 8 h post-infection (2 h: t(4) = 4.067, P = 0.015; 4 h: t(4) = 10.67, P = 0.0004; 8 h: t(4) = 13.39, P = 0.0002; 12 h: t(4) = 8.333, P = 0.0011) (Additional file 2: Figure S2). The gene discussed is IL1B; the disease is infection.