In order to provide a more comprehensive scenario through which estrogens may trigger the transcription of CYP1B1 and its metabolic activity in a feed-forward manner, we have ascertained that estrogenic GPER signaling regulates CYP1B1 expression in ER-negative and GPER-positive breast cancer cells, CAFs obtained from breast cancer patients and CAFs derived from a cutaneous metastasis of an invasive mammary ductal carcinoma (met-CAFs). The gene discussed is GPER1; the disease is breast cancer.