SP1 and Miyoshi myopathy: WB analysis indicated that transfection of EZH2-targeting siRNAs in JJN3 and AMO-BZB cells triggered downregulation of validated miR-29b targets, such as SP1, CDK6, and to a lesser extent MCL-1 (Figure 2D), indicating that EZH2-depletion induced a functionally active miR-29b; downregulation of SP1, CDK6 and MCL-1 was also achieved after treatment of MM cells with EZH2 inhibitors (Figure 2E).