Based on these results, we cultured B-ALL cells in our optimized culture medium, referred to as FI76V, containing IMDM, 1% BIT, FLT3L (20 ng/ml), IGF-1 (10 ng/ml), IL-7 (10 ng/ml) and IL-6 (10 ng/ml) on VCAM1 pre-coated wells and evaluated the ability of the media to expand primary human B-ALL cells. Here, IGF1 is linked to acute lymphoblastic leukemia.