To determine the consequence of suppression of H2B Y37-phosphorylation, various melanoma (SK-MEL-2, WM1363, MO5, IPC298 and WM1366), prostate (LNCaP-C4 and LAPC4), GBM (T98G) and HEK293 cells were treated with AZD1775 and the effect on cell proliferation was assessed (Figure 1E). The gene discussed is H2BC21; the disease is melanoma.