To determine whether WEE1 mediated IDH2 transcriptional suppression also impacts 5-hmC levels in different cancer models, melanoma (SBC12, WM1366, WM38, SK-MEL-2, B16, IPC298 and MO5), GBM (T98G) and prostate cancer cells (LAPC4 and 22RV1) were treated with AZD1775 and total genomic DNA was extracted to analyze the 5-hmC status. The gene discussed is WEE1; the disease is prostate cancer.