To investigate whether WEE1/pY37-H2B signaling plays a direct role in regulating IDH2 gene transcription, various melanoma (WM1963, IPC298, WM1396, WM3918, SK-MEL-2, WM38 and WM1366), prostate (LNCaP and LAPC4), GBM (U87) and HEK293 cells were treated with WEE1-specific inhibitor, AZD1775. The gene discussed is H2BC21; the disease is glioblastoma.