WEE1 and prostate carcinoma: To examine the presence of pY37-H2B marks within the human IDH2 gene locus, melanoma derived cell lines IPC298 and SK-MEL2, prostate cancer derived cell lines- LAPC4 and GBM derived cell line T98G were treated with WEE1 kinase inhibitor AZD1775 and sheared chromatin was prepared.