In GBM-derived stem-like cell-enriched 51A, established and maintained in DMEM/F12 containing growth factors including EGF, the anti-EGFR and anti-NOTCH effects were shown after a long-term exposure to ZR30, with reductions of both EGFR and NOTCH1 (a decrease of 58% and 82%, respectively) levels, compared to the untreated cells (Figure 2E). This evidence concerns the gene NOTCH1 and glioblastoma.