MMP2 and glioblastoma: Using the gelatin zymography assay for quantification of the level of active gelatinase (here MMP2 based on the size of 67 kD) in conditioned medium of cell culture (see supplementary text for the methods details), we analyzed the effect of ZR30 on the regulation of MMP2 activation in three high-MMP2-expressing GBM cell lines (LN229, U87, and T98G) and a GBM primary culture (51B).