Data presented above showed reproducible production of an in vitro synthesized protein ZR30, based on the sequence of a human fibulin-3 protein variant, ETSP, omitting the N-terminal signal peptide for extracellular export, and its tumor suppressive effects by targeting the same oncogenic proteins (MMP2, EGFR, NOTCH1) and AKT-mediated signaling pathway in GBM cell lines, as shown by overexpression of ETSP. The gene discussed is EGFR; the disease is glioblastoma.