However, immunoblot data (top panel) showed a significantly increased amount of Bax (~23 kDa without smaller, degraded products) in cytoplasm (data not shown) or mitochondria of AD brain tissues compared to controls (Fig. 3A, bottom panel), suggesting that Bax likely plays a role in mitochondria-dependent apoptosis of neuronal cells in AD individuals, as similar to the cultured cells [16]. This evidence concerns the gene BAX and Alzheimer disease.