Supplementary Figure S2b, c shows the gating strategy for the CD45RA−CD45RO+ CD4+ Th cell subsets: Th1 (CXCR3+CCR4−CCR6−), Th2 (CCR4+CCR6−CXCR3−), Th17 (CCR4+CCR6+CXCR3−) and Th17.1 (CXCR3+CCR4−CCR6+) as applied to a healthy control and a pre-hepatitis sample. This evidence concerns the gene CCR4 and hepatitis A virus infection.