In the present study, we demonstrated that CXCL8 derived from TAM-like PBMo-derived macrophages induced cell migration and invasion by indirect co-culture with ESCC cell lines, and these were significantly suppressed by neutralizing antibodies against CXCR1, CXCR2 or CXCL8. This evidence concerns the gene CXCL8 and esophageal squamous cell carcinoma.