We confirmed the expressions of CXCR1 and CXCR2 (which are CXCL8 receptors) on the ESCC cell lines (TE-8, TE-9 and TE-15) by RT-PCR (Figure 2A) and western blotting (Figure 2B), respectively. The gene discussed is CXCR2; the disease is esophageal squamous cell carcinoma.