To directly evaluate the effects of FAM3C-HSF1-CaM pathway on hepatic gluconeogenesis and hyperglycemia of type 1 diabetic mice, FAM3C or HSF1 was overexpressed in STZ-treated mouse livers via tail vein injection of Ad-FAM3C or HSF1 plasmid. Here, CALM1 is linked to type 1 diabetes mellitus.