To elucidate whether cross linking activity of J2 is specific for HSP27, HSP27 high expressed lung cancer cells, NCI-H460 and HSP27 low expressed lung cancer cells, NCI-H1299 cells was compared with HSP27 cross linking activity of J2 and found that J2 showed stronger cross linking activity in HSP27 high expressed lung cancer cells and J2 was more profound activity than SW15 (Figure 1C). Here, HSPB1 is linked to lung carcinoma.