IHC findings were observed according to tumor differentiation in tissue microarrays (TMA) from an independent cohort of patients (Figure 1C, d-h); CTHRC1 expression was barely detected in normal tissue (Figure 1C, d), but moderate and strong expression was seen in HCC with poor differentiation (Figure 1C, e-h). This evidence concerns the gene CTHRC1 and hepatocellular carcinoma.